tubes 14 × 89 mm open top thinwall ultraclear tubes Search Results


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trimethoprim sulfamethoxazole  (Chem Impex International)
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ultra clear sw 41 tubes  (Beckman Coulter)
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eef63672 1  (ATCC)
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recombinant human pro-mmp-14 (catalytic domain, amino acids 89–265)  (Merck KGaA)
90
Merck KGaA recombinant human pro-mmp-14 (catalytic domain, amino acids 89–265)
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work jme337 dh5α pimr83 14 y lipolytica strains w29 mat 3  (ATCC)
95
ATCC work jme337 dh5α pimr83 14 y lipolytica strains w29 mat 3
Strains, plasmids, and oligonucleotides used in this study
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2 amino 2 methyl 1 propanol  (Thermo Fisher)
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Strains, plasmids, and oligonucleotides used in this study
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sw41 rotor beckman ultra clear centrifuge tubes  (Beckman Coulter)
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Beckman Coulter sw41 rotor beckman ultra clear centrifuge tubes
Strains, plasmids, and oligonucleotides used in this study
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dna extraction method  (5 PRIME)
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5 PRIME dna extraction method
Strains, plasmids, and oligonucleotides used in this study
Dna Extraction Method, supplied by 5 PRIME, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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benzoic anhydride  (Chem Impex International)
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Strains, plasmids, and oligonucleotides used in this study
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matrix  (GE Healthcare)
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GE Healthcare matrix
Strains, plasmids, and oligonucleotides used in this study
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Image Search Results


Strains, plasmids, and oligonucleotides used in this study

Journal:

Article Title: Characterization of an Extracellular Lipase Encoded by LIP2 in Yarrowia lipolytica

doi:

Figure Lengend Snippet: Strains, plasmids, and oligonucleotides used in this study

Article Snippet: Media were buffered with 50 mM phosphate buffer, pH 6.8. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain (host strain) or primer Plasmid, genotype, or sequence (5′→3′) c Reference, source or location (bp) E. coli strains JME225 (DH5α) pKS- LIP2 W29a This work JME257 (DH5α) JMP5 ( ura3d1 ) This work JME259 (DH5α) JMP6 ( ura3d4 /pPOX2- LIP2 ) This work JME276 (DH5α) JMP3 ( ura3d4 ) This work JME260 (DH5α) JMP8 ( ura3d1 /pPOX2- LIP2 ) This work JME298 (DH5α) JMP13 ( ura3d1 /pLIP2- LIP2 ) This work JME300 (DH5α) JMP14 ( ura3d4 /pLIP2- LIP2 ) This work JME337 (DH5α) pIMR83 14 Y. lipolytica strains W29 MAT 3 (CLIB 89, ATCC 20460) a POld MAT a ura3-302 leu2-270 xpr2-322 3 (CLIB 139) E150 MAT a ura3-302 leu2-270 xpr2-322 his1 3 (CLIB 122) Y. lipolytica recombinant strains b JMY184 = POld-6-15 JMP6/pPOX2- LIP2 A This work JMY279 = POld-6-17 JMP6/pPOX2- LIP2 A This work JMY277 = POld- lip2 KO p lip2 KO lip2 KO This work JMY278 = POld-6-15- xpr6 KO p xpr6 KO/pPOX2- LIP2 A xpr6 KO This work JMY281 = POld-14-1 JMP14/pLIP2- LIP2 A This work JMY282 = POld-14-2 JMP14/pLIP2- LIP2 A This work JMY283 = E150-6-2 JMP6/pPOX2- LIP2 A This work Primers YlipSA GCi CCi CCi AGR GAR TGi CC 4313 YlipATG CCC GGG CCG CAG TGG CCA CAA TG A AGC TT T CCA CCA TCC TTT TCA CAG CC 3733 YlipSTOP GTT G GC GGC CGC GAA TTC GGA TCC TAA ATA AAC GAT ATT CAT TTA TTA AAG TAG ATA GTT GAG G 4806 Open in a separate window a CLIB, Collection de Levures d'Intérêt Biotechnologique, Thiverval-Grignon, France. b Transformants were named according to the strain and plasmid used, and transformants were numbered as follows: for example; POld1-6-1 (strain-plasmid-clone number) c Underlined sequences correspond to introduced restriction enzyme sites as follows: for YlipATG, Hin dIII; for YlipSTOP, Not I, Eco RI, and Bam HI.

Techniques: Plasmid Preparation, Sequencing, Recombinant